Electrochemiluminescent aptasensor based on β-cyclodextrin/graphitic carbon nitride composite for highly selective and ultrasensitive assay of platelet derived growth factor BB

Abstract Here, an electrochemiluminescent aptasensor based on β-cyclodextrin/graphitic carbon nitride (β-CD/g-C3N4) composite for selective and highly ultrasensitive assay of platelet derived growth factor BB (PDGF-BB) is fabricated. To achieve the sensitive and specific detection of PDGF-BB, it is an available strategy to explore excellent electrochemiluminescent (ECL) luminophores with the advantages of high electrochemical-driven luminescent activity and a specific recognition function. In this work, for the first time a novel β-CD non-covalently functionalized g-C3N4 composite β-CD/g-C3N4 was employed to construct the ECL detection platform for PDGF-BB based on its aptamer. The limit of detection was determined to be as low as 2.6 × 10−13 g/mL. It was observed that β-CD was playing a key role in improving the adamantane-labeled DNA amount modified on the electrode surface. It can further help to amplify the ECL quenching effect by both energy transfer and photo-excited electron transfer processes between g-C3N4 emitter and ferrocene quencher. It is anticipated that β-CD/g-C3N4 may be a class of promising material for constructing ECL sensors.