电化学发光
鲁米诺
石墨烯
猝灭(荧光)
检出限
氧化物
化学
荧光
光化学
核化学
分析化学(期刊)
材料科学
纳米技术
色谱法
有机化学
物理
量子力学
作者
Xiaojian Li,Lu Peng,Bin Wu,Yaoguang Wang,Huan Wang,Bin Du,Xuehui Pang,Qin Wei
标识
DOI:10.1016/j.bios.2018.04.030
摘要
A novel electrochemiluminescence (ECL) signal-off strategy based on CuS in situ grown on reduced graphene oxide (CuS-rGO) quenching luminol/H2O2 system was firstly proposed. Luminol was grafted on the surface of [email protected]3O4-Cu3(PO4)2 nanoflowers ([email protected]3O4-Cu3(PO4)2) which exhibited excellent catalytic effect towards the reduction of H2O2 to enhance the ECL intensity of luminol. Cu3(PO4)2 nanoflowers showed large surface area which can immobilize more Fe3O4 and Au nanoparticles. The quenching mechanism of CuS-rGO was due to ECL resonance energy transfer (RET). The spectral overlap between fluorescence spectrum of [email protected]3O4-Cu3(PO4)2 and UV–vis absorption spectrum of CuS-rGO revealed that resonance energy transfer was possible. Au nanoparticles were immobilized on the surface of CuS-rGO to capture secondary antibodies. After a sandwich-type immunoreaction, a remarkable decrease of ECL signal was observed. Under the optimal conditions, the immunosensor showed excellent performance for N-terminal pro-brain natriuretic peptide (NT-proBNP) detection with a wide detection range from 0.5 pg mL−1 to 20 ng mL−1 and a low detection limit of 0.12 pg mL−1 (S/N = 3). The prepared NT-proBNP immunosensor displayed high sensitivity, excellent stability and good specificity.
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