纳米孔
电动现象
微通道
微流控
电泳
介电泳
化学
电场
俘获
膜
纳米技术
纳米孔
光电子学
分析化学(期刊)
材料科学
色谱法
生态学
生物化学
物理
量子力学
生物
作者
Michelle L. Kovarik,Stephen C. Jacobson
摘要
We report integrated nanopore/microfluidic devices in which the unique combination of low pore density, conical nanopore membranes with microfluidic channels created addressable, localized high-field regions for electrophoretic and dielectrophoretic trapping of particles. A poly(ethylene terephthalate) track-etched membrane containing conical pores ∼130 nm in diameter at the tip and ∼1 μm in diameter at the base was used as an interconnect between two perpendicular poly(dimethylsiloxane) microfluidic channels. Integration of the nanopore membrane with microfluidic channels allowed for easy coupling of the electrical potentials and for directed transport of the analyte particles, 200 nm and 1 μm polystyrene microspheres and Caulobacter crescentus bacteria, to the trapping region. Square waves applied to the device generated electric field strengths up to 1.3 × 105 V/cm at the tips of the nanopores in the microchannel intersection. By varying the applied potentials from ±10 to ±100 V and exploring frequencies from dc to 100 kHz, we determined the contributions of electrophoretic and dielectrophoretic forces to the trapping and concentration process. These results suggest that tunable filter elements can be constructed in which the nanoporous elements provide a physical barrier and the applied ac field enhanced selectivity.
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