Simultaneous in Situ Total Internal Reflectance Fluorescence/Atomic Force Microscopy Studies of DPPC/dPOPC Microdomains in Supported Planar Lipid Bilayers

In situ simultaneous total internal reflectance fluorescence and in situ scanning probe microscopy performed on a phase-segregated supported planar lipid bilayer enabled direct in situ real-time correlated topographical and fluorescence images of nanometer-sized gel and fluid-phase lipid domains, presaging future in situ studies of membrane protein assemblies by single molecule imaging.