生物
血管生成
免疫组织化学
表型
病理
免疫荧光
内皮干细胞
内皮
细胞生物学
抗体
癌症研究
免疫学
体外
内分泌学
基因
医学
生物化学
作者
Manuela Mura,Roy Swain,Xiaodong Zhuang,Henrik Vorschmitt,Gary Reynolds,Sarah M. Durant,James Beesley,John Herbert,Helen Sheldon,Maud Andre,Sharon Sanderson,Katie E. Glen,Nguyet-Thin Luu,Helen M. McGettrick,Philipp Antczak,Francesco Falciani,Gerard B. Nash,Zsuzsanna Nagy,Roy Bicknell
出处
期刊:Oncogene
[Springer Nature]
日期:2011-06-27
卷期号:31 (3): 293-305
被引量:100
摘要
Tumor endothelial markers (TEMs) that are highly expressed in human tumor vasculature compared with vasculature in normal tissue hold clear therapeutic potential. We report that the C-type lectin CLEC14A is a novel TEM. Immunohistochemical and immunofluorescence staining of tissue arrays has shown that CLEC14A is strongly expressed in tumor vasculature when compared with vessels in normal tissue. CLEC14A overexpression in tumor vessels was seen in a wide range of solid tumor types. Functional studies showed that CLEC14A induces filopodia and facilitates endothelial migration, tube formation and vascular development in zebrafish that is, CLEC14A regulates pro-angiogenic phenotypes. CLEC14A antisera inhibited cell migration and tube formation, suggesting that anti-CLEC14A antibodies may have anti-angiogenic activity. Finally, in endothelial cultures, expression of CLEC14A increased at low shear stress, and we hypothesize that low shear stress due to poor blood flow in the disorganized tumor vasculature induces expression of CLEC14A on tumor vessels and pro-angiogenic phenotypes.
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