抗菌剂
食品科学
玉米浆
豆粕
淀粉
发酵
化学
响应面法
生物技术
生物
色谱法
有机化学
原材料
作者
Yoon-Jung Ju,Kwang‐Hee Son,Chun-Zhi Jin,Byung Kook Hwang,Dong-Jin Park,Chang‐Jin Kim
标识
DOI:10.1007/s10068-017-0257-1
摘要
The nutritional requirements for antimicrobial activity of Streptomyces rimosus AG-P1441 were optimized using statistically-based experimental designs at a flask level. Based on a one-factor-at-a-time (OFAT) approach, glucose, corn starch and soybean meal were identified as the carbon and nitrogen sources having a significant effect on antimicrobial productivity. As a result of investigating the effect of glucose concentration, the highest antimicrobial activity was observed at 3% concentration. Response surface methodology (RSM) was then applied to optimize the growth medium components (corn starch, soybean meal, MgCl2 and glutamate). Antimicrobial productivity increased sharply when the medium consisted of 3% glucose, 3.5% corn starch, 2.5% soybean meal, 1.2 mM MgCl2 and 5.9 mM glutamate. The fermentation using optimized culture medium in a 5-L bioreactor allowed a significant increase in antimicrobial activity, evaluated by the paper disc assay, revealed a 29 mm inhibition zone diameter against Phytophthora capsici.
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