胆绿素
胆绿素还原酶
血红素加氧酶
胆红素
血红素
同工酶
生物化学
化学
还原酶
血红素蛋白
酶
分子生物学
生物
内分泌学
标识
DOI:10.1002/0471140856.tx0903s00
摘要
Abstract It is possible to detect changes in the level of expression of the two isozymes of heme oxygenase (HO). The sequence differences allow the production of isozyme‐specific antibodies that can be used in immunoblot analysis. Total HO activity in tissue microsomal fractions or extracts of bacteria transformed with an HO gene can be measured as described in this unit using a coupled assay employing biliverdin reductase, which converts the biliverdin product to bilirubin. Bilirubin is then quantified with a scanning spectrophotometer.
科研通智能强力驱动
Strongly Powered by AbleSci AI