琼脂糖
分子生物学
生物
DNA
聚合酶链反应
甲藻
连接酶连锁反应
琼脂糖凝胶电泳
底漆二聚体
基因
生物化学
多重聚合酶链反应
植物
作者
Irma Marı́n,Ángeles Aguilera,B. Reguera,José P. Abad
出处
期刊:BioTechniques
[Future Science Ltd]
日期:2001-01-01
卷期号:30 (1): 88-93
被引量:56
摘要
A method is described to prepare total DNA from single cells of dinoflagellates, which can be used for PCR amplification. As model organisms, we used a stock strain of Alexandrium catenella and cells of Dinophysis acuminata harvested from the Atlantic Ocean. Fresh grown cells or cells maintained in different preservatives were tested as sources for DNA preparation. The method used to prepare DNA combines physicochemical and enzymatic procedures on cells embedded in agarose plugs or beads. The agarose pieces containing the DNA were used to perform PCR amplification of a fragment of DNA containing a 5.8S rRNA gene and the flanking internal transcribed spacers (ITS1 and ITS2).
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