表面等离子共振
适体
唾液
检出限
胶体金
化学
材料科学
分析物
纳米技术
纳米颗粒
色谱法
分子生物学
生物
生物化学
作者
Seongjae Jo,Wonseok Lee,Joohyung Park,Woong Kim,Woochang Kim,Gyudo Lee,Heon Jeong Lee,Junghwa Hong,Jinsung Park
标识
DOI:10.1016/j.snb.2019.127424
摘要
Cortisol, which is a stress biomarker that causes various mental disorders, is found in sweat, saliva, blood, and urine. Because mental disorders resulting from stress are a silent contributor to various diseases, the detection of cortisol level is an important step in their prevention. Here, we developed a highly sensitive, localized surface plasmon resonance (LSPR) aptasensor for the direct detection of cortisol in saliva. The LSPR aptasensor was constructed using gold nanoparticles of different sizes. Subsequently, the surface of the LSPR substrate was immobilized using an aptamer for capturing cortisol. The detection capability of the LSPR aptasensor was compared with that of the antibody- immobilized sensor. The LSPR aptasensor displayed excellent detection capability for a wide range of cortisol concentrations extending from 0.1–1000 nM with a detection limit of 0.1 nM. In addition, the LSPR aptasensor was able to selectively detect cortisol among other substances with similar chemical structures (e.g., cortisone, corticosterone, progesterone, and triamcinolone). These results indicate that the LSPR aptasensor may be used for the highly sensitive and selective detection of cortisol in salivary samples.
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