线粒体DNA
线粒体
细胞
计算生物学
DNA
生物化学
化学
生物
细胞生物学
生物物理学
基因
作者
Juan C. Landoni,Liya Wang,Anu Suomalainen
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 143-151
标识
DOI:10.1007/978-1-0716-1266-8_10
摘要
Deoxynucleoside 5′-triphosphates (dNTPs) are the molecular building blocks for DNA synthesis, and their balanced concentration in the cell is fundamental for health. dNTP imbalance can lead to genomic instability and other metabolic disturbances, resulting in devastating mitochondrial diseases.The accurate and efficient measurement of dNTPs from different biological samples and cellular compartments is vital to understand the mechanisms behind these diseases and develop and scrutinize their possible treatments. This chapter describes an update on the most recent development of the traditional radiolabeled polymerase extension method and its adaptation for the measurement of whole-cell and mitochondrial dNTP pools from cultured cells and tissue samples. The solid-phase reaction setting enables an increase in efficiency, accuracy, and measurement scale.
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