The thickening and modification of the galactan-enriched layer during primary phloem fibre development in Cannabis sativa

Abstract Primary phloem fibres (PPFs) have higher fibre quality and are economically more important for the textile sector than secondary phloem fibres. Both the chemical composition and mechanical structure of the secondary cell wall mainly influence the quality of bast fibres. We investigated the thickening of the galactan-enriched (Gn) layer and its modification process into a gelatinous (G)-layer, which is the largest portion of the secondary cell wall, during the development of the PPF in Cannabis sativa. Stem segments of hemp collected at 17, 29, 52 and 62 days after sowing were comparatively examined using light microscopy, scanning electron microscopy and transmission electron microscopy. The initial cells of PPF started the proliferation and differentiation at 17 days, but the secondary cell wall thickening had already commenced before the 29 days. Both the G- and Gn-layer were rapidly added onto the S-layer of PPFs; thus, the secondary cell wall thickness increased approximately 2-fold at 52 days (from the 29-day mark), and 8-fold at 62 days. The cortical microtubule arrays appeared adjacent to the plasma membrane of PPF cells related to the cellulose synthesis. Additionally, cross-sectioned microfibrils were observed on Gn-layer as the cluster of tiny spots. At 62 days, the specific stratification structure consisting of several lamellae occurred on the G-layer of the secondary cell wall. The secondary cell wall thickened remarkably at 52 days through 62 days so that the mature secondary cell wall consisted of three distinctive layers, the S-, G- and Gn-layer. Cortical microtubule arrays frequently appeared adjacent to the plasma membrane together with cellulose microfibrils on secondary cell wall. The G-layer of PPF at 62 days exhibited the characteristic stratification structure, which demonstrates the modification of the Gn-layer into the G-layer.