High Throughput Blood Analysis Based on Deep Learning Algorithm and Self‐Positioning Super‐Hydrophobic SERS Platform for Non‐Invasive Multi‐Disease Screening

Abstract Blood analysis is crucial for early cancer screening and improving patient survival rates. However, developing an effective strategy for early cancer detection using high‐throughput blood analysis is still challenging. Herein, a novel automatic super‐hydrophobic platform is developed together with a deep learning (DL)‐based label‐free serum and surface‐enhanced Raman scattering (SERS), along with an automatic high‐throughput Raman spectrometer to build an effective point‐of‐care diagnosis system. A total of 695 high‐quality serum SERS spectra are obtained from 203 healthy volunteers, 77 leukemia M5, 94 hepatitis B virus, and 321 breast cancer patients. Serum SERS signals from the normal ( n = 183) and patient ( n = 443) groups are used to assess the DL model, which classify them with a maximum accuracy of 100%. Furthermore, when SERS is combined with DL, it exhibits excellent diagnostic accuracy (98.6%) for the external held‐out test set, indicating that this method can be used to develop a high throughput, rapid, and label‐free tool for screening diseases.