荧光
胆固醇氧化酶
检出限
催化作用
基质(水族馆)
化学
材料科学
核化学
色谱法
纳米技术
胆固醇
有机化学
生物化学
物理
海洋学
量子力学
地质学
作者
Wittaya Ngeontae,Kitayanan Ponlakhet,Samuch Phetduang,Nopphakon Phongsanam,Supinya Nijpanich,Thirakan Phongsraphang,Kessarin Ngamdee
标识
DOI:10.1016/j.foodchem.2024.139116
摘要
A new hybrid biological-chemical catalyst, magnetic nanoparticles functionalized with cholesterol oxidase (Fe3O4/APTES/ChOx), was developed for cholesterol detection. In the presence of cholesterol, the enzyme produced H2O2, which facilitated the generation of fluorescent molecules from the fluorogenic substrate with the assistance of Fe3O4 nanoparticles. A smartphone camera with a miniature fluorescent apparatus was used to assess fluorescence emission. Then, a smartphone application was employed to translate the fluorescence intensity to the red, green, and blue (RGB) domain. The developed approach achieved excellent selectivity and acceptable performances while supporting an onsite analysis approach. The practical operational range spanned from 5 to 100 nM, with a detection limit of 0.85 nM. Fe3O4/APTES/ChOx was applied for up to four replicates of reuse and demonstrated stability for at least 30 days. The applicability of the method was evaluated in milk samples, and the results were in accordance with the reference method.
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