已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

A Ca2+ sensor BraCBL1.2 involves in BraCRa-mediated clubroot resistance in Chinese cabbage

克鲁布罗特 生物 拟南芥 转录组 基因 过敏反应 毒力 接种 十字花科 微生物学 植物抗病性 植物 遗传学 基因表达 园艺 芸苔属 突变体
作者
Yinglan Piao,Shizhen Li,Y. Chen,Sisi Zhao,Zhongyun Piao,Haiping Wang
出处
期刊:Horticulture research [Nature Portfolio]
卷期号:11 (1) 被引量:1
标识
DOI:10.1093/hr/uhad261
摘要

Clubroot disease caused by Plasmodiophora brassicae (P. brassicae) severely threatens the cultivation of Cruciferous plants, especially Chinese cabbage. Recently, resistance genes in plants have been reported to encode for a Ca2+-permeable channel in the plasma membrane, which can mediate the cytosolic Ca2+ increase in plant cells upon pathogen attack. However, the downstream Ca2+ sensor and decoder are still unknown. In this study, we identified the virulent and avirulent P. brassicae isolates (Pbs) of two near isogenic lines, CR 3-2 and CS 3-2, with CR 3-2 harboring clubroot resistant gene BraCRa. The transcriptomic analysis was then conducted with CR 3-2 after inoculating with virulent isolate PbE and avirulent isolate Pb4. From the differentially expressed genes of transcriptomic data, we identified a Ca2+-sensor encoding gene, BraCBL1.2, that was highly induced in CR 3-2 during infection by Pb4 but not by PbE. Moreover, GUS histochemical staining and subcellular localization analysis revealed that BraCBL1.2 was specifically expressed in the root hair cells of Arabidopsis and encoded a putative Ca2+ sensor localized in the plasma membrane. We also developed an assay to investigate the BraCRa-mediated hypersensitive response (HR) in tobacco leaves. The results suggest that BraCBL1.2 is involved in the BraCRa-mediated plant ETI immune response against P. brassicae. In addition, we verified that overexpression of BraCBL1.2 enhanced clubroot resistance in Arabidopsis. Collectively, our data identified the involvement of a Ca2+ sensor in BraCRa-mediated clubroot resistance in Chinese cabbage, providing a theoretical basis for further research on the resistance of Chinese cabbage to P. brassicae.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
NexusExplorer应助思辨233采纳,获得10
1秒前
我是老大应助tosania采纳,获得30
1秒前
拾壹完成签到,获得积分10
3秒前
烟花应助粪海狂蛆采纳,获得10
3秒前
大模型应助怡然凌柏采纳,获得10
4秒前
辰星未湮完成签到,获得积分10
5秒前
氟马西尼完成签到,获得积分10
6秒前
沈沈完成签到 ,获得积分10
6秒前
独特跳跳糖完成签到 ,获得积分10
7秒前
soumei完成签到,获得积分10
8秒前
9秒前
jimskylxk发布了新的文献求助10
10秒前
15秒前
15秒前
平常万言发布了新的文献求助10
16秒前
科研通AI2S应助友好的南霜采纳,获得10
17秒前
天津中医药峰完成签到,获得积分10
18秒前
18秒前
赘婿应助jimskylxk采纳,获得10
19秒前
Walden完成签到,获得积分10
19秒前
Miller发布了新的文献求助10
20秒前
共享精神应助方春采纳,获得30
20秒前
善学以致用应助袁寒烟采纳,获得10
21秒前
Autumn Ze发布了新的文献求助10
21秒前
xiaopacai完成签到 ,获得积分10
22秒前
22秒前
YW发布了新的文献求助10
22秒前
WYP发布了新的文献求助10
23秒前
orixero应助Walden采纳,获得10
24秒前
24秒前
脑洞疼应助开心采纳,获得10
24秒前
28秒前
晓槐发布了新的文献求助10
28秒前
28秒前
29秒前
jimskylxk完成签到,获得积分10
29秒前
bkagyin应助孙杰采纳,获得10
31秒前
平常万言发布了新的文献求助10
33秒前
ding应助heart采纳,获得10
33秒前
高分求助中
【重要!!请各位用户详细阅读此贴】科研通的精品贴汇总(请勿应助) 10000
Plutonium Handbook 1000
Three plays : drama 1000
International Code of Nomenclature for algae, fungi, and plants (Madrid Code) (Regnum Vegetabile) 1000
Semantics for Latin: An Introduction 999
Psychology Applied to Teaching 14th Edition 600
Robot-supported joining of reinforcement textiles with one-sided sewing heads 600
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4098177
求助须知:如何正确求助?哪些是违规求助? 3635935
关于积分的说明 11524564
捐赠科研通 3346066
什么是DOI,文献DOI怎么找? 1839021
邀请新用户注册赠送积分活动 906461
科研通“疑难数据库(出版商)”最低求助积分说明 823699