检出限
荧光
化学
硒
谷胱甘肽
线性范围
谷胱甘肽过氧化物酶
生物化学
色谱法
酶
有机化学
物理
量子力学
作者
Caimei Wu,Yuwei Zhang,Min–Koo Han,Ruinan Zhang,Hua Li,Fali Wu,Aimin Wu,Xianxiang Wang
标识
DOI:10.1016/j.foodchem.2023.137147
摘要
Developing rapid monitoring methods to detect antibiotic residues in food plays an important role in safeguarding human health. This study presents the development of a novel fluorescence-enhanced detection method for chlortetracycline (CTC) using a GSH-Se nanozyme. A GSH-Se nanozyme prepared using a one-pot hydrothermal method not only possesses excellent fluorescent properties but also exhibits good glutathione peroxidase-like activity. The results show that the addition of CTC leads to a significant enhancement in the fluorescence intensity of GSH-Se, and this increase exhibits a good linear relationship with the concentration of CTC. The linear range of this method is 0.02–1 µM, and the limit of detection (LOD) for CTC was 0.02 µM. Moreover, the cell toxicity of GSH-Se is low and can be used for monitoring and imaging of CTC in cells, and satisfactory results have been obtained in the analysis of actual food samples.
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