ANTIMICROBIAL EFFICACY OF ENDOSEQUENCE BIOCERAMIC SEALER INCORPORATED WITH SILVER AND CHITOSAN NANOPARTICLES (AN IN-VITRO STUDY)

Introduction:The present study evaluated the antibacterial efficiency of Endosequence bioceramic sealer (BC) and with the addition of silver and chitosan nanoparticles (NPs).Methods: Thirty extracted human maxillary anterior teeth were sectioned horizontally at the level of cemento-enamel junction.The teeth were randomly divided into three equal groups (10 teeth each) according to the material used; Group 1: Endosequence BC, Group 2: Endosequence BC + Silver NPs and Group 3: Endosequence BC + Chitosan NPs.Silver and chitosan NPs were synthesized and characterized then, pastes of both NPs were prepared.Root canal enlargement was performed.C linical isolate of E. faecalis was inoculated.Endosequence BC sealer was used according to manufacturer's instructions.Silver NP and Endosequence BC sealer were combined by adding nanoparticles of 2.3% by volume.Chitosan NP was used at 15% by volume.Results: There was a statistically significant difference between EndoSequence BC as well as EndoSequence BC+Silver NPs groups and EndoSequence BC+Chitosan NPs group (p=0.007).No significant difference was recorded between EndoSequence BC and EndoSequence BC+Silver NPs groups (p>0.05).A significant increase in bacterial growth was observed for EndoSequence BC+Chitosan NPs (0.19±0.01), then EndoSequence BC (0.18±0.01), with least bacterial growth in EndoSequence BC+Silver NPs (0.17±0.01).All tested materials had bactericidal effect and eliminated E. faecalis on trypticase soy agar.Conclusions: Addition of silver and chitosan NPs to Endosequence BC sealer did not significantly increase its anti-microbial efficacy against E. fecalis.