采后
抑制因子
热休克蛋白
活性氧
热冲击系数
心理压抑
发起人
细胞生物学
转录因子
电泳迁移率测定
热冲击
生物
基因
化学
生物化学
植物
基因表达
热休克蛋白70
作者
Jia Si,Bing-bing Ye,Zong-li Liu,Xian-mei Xiao,Yingying Yang,Zhengqiu Fan,Wei Shan,Jian‐fei Kuang,Wang‐jin Lu,Xiaolu Su,Jianye Chen,Wei Wei
标识
DOI:10.1016/j.postharvbio.2022.112056
摘要
Both heat shock factors (Hsfs) and reactive oxygen species (ROS) are widely perceived to be involved in chilling stress response. However, the particular mechanism of Hsfs mediated-ROS metabolism related to chilling stress remains largely unknown, especially during chilling injury development of postharvest economically cold-sensitive fruit such as banana. In this study, we found that heat shock treatment (HST) could effectively alleviate postharvest banana chilling injury and reduce ROS accumulation during storage. The expression levels of several respiratory burst oxidase homolog (RBOH) genes, encoding ROS-producing enzymes, increased consistently with the progression, whereas were inhibited upon HST. Significantly, we identified an Hsfs MaHsf26, which exhibited a opposite expression pattern as that of MaRBOHs, as the putative binding protein of MaRBOHB-like1, MaRBOHE-like and MaRBOHF-like promoters using yeast one-hybrid (Y1H) screening analysis. Further electrophoretic mobility shift assay (EMSA) and dual luciferase reporter (DLR) analysis displayed MaHsf26 suppressed the transcriptions of MaRBOHB-like1, MaRBOHE-like and MaRBOHF-like through binding to the heat shock elements (HSE) in their promoters, respectively. In general, our results suggested that MaHsf26 plays as a transcriptional repressor of ROS generation by suppressing the transcriptions of MaRBOHs and thereby involving in the HST-mitigated chilling injury in postharvest banana fruit.
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