Analysis of chemical and blood transition components of Xipayi Maizibizi Oral Liquid and its excitatory purine regulation-mechanism study in treating overactive bladder

Abstract Objective To explore the chemical components and blood transition components of Xipayi Maizibizi Oral Liquid (XP) as well as the efficacy and mechanism of XP in the treatment of overactive bladder (OAB). Methods The chemical components and blood transition components were analysed. Rats were undergone bladder outlet obstruction surgery and divided into five groups. The study observed the general conditions, urodynamic argument and the bladder histopathological changes. The expression levels of ATP and P2X3 were evaluated. Results One hundred and eighty-six chemical components were identified in XP, including 26 phenolic acids, 37 flavonoids, 7 quinones, 11 lignans and coumarins, 8 tannins, 9 alkaloids, 37 terpenoids, 32 steroids, and 19 other components. One hundred and twenty-eight components of XP were found in the blood, including 23 phenolic acids, 22 flavonoids, 3 quinones, 8 lignans and coumarins, 7 tannins, 7 alkaloids, 21 terpenoids, 20 steroids, and 17 other components. XP demonstrated effective treatment of OAB and downregulated the expression of ATP and P2X3. Conclusion The characterization of the chemical composition and blood transition components of XP provided a foundation for further pharmacodynamic material basis and quality control of XP. XP could enhance the pathological status of rats with OAB by regulating ATP/P2X3.