Application of electrospun membranes of polylactic acid and polypyrrole as a biosensor for the detection of cholesterol

Background: Hydrogen peroxide (H 2 O 2 ) plays a crucial role in various industries and enzymatic reactions, including cholesterol oxidation. Cholesterol, vital for physiological functions, can lead to cardiovascular and hepatic diseases when present in excess. Accurate detection is crucial, yet current techniques are costly and time-consuming. Biosensors offer a promising alternative due to their sensitivity, speed, and portability in detecting H 2 O 2 . Objective: This study aims to develop a sensitive, simple, rapid, and cost-effective biosensor for H 2 O 2 detection using electrospun membranes coated with polypyrrole (PPy). Methods: Poly(lactic acid) (PLA) membranes were prepared using the electrospinning technique. Subsequently, these membranes were coated with polypyrrole (PPy) through in situ chemical polymerization. The obtained materials were characterized using SEM, contact angle measurements, XPS, and their electrical properties were analyzed. Results : PLA/PPy composite membranes exhibited electrical conductivities on the order of 10 −2 S cm −1 . Upon exposure to H 2 O 2 and enzymatic reaction, a significant decrease in their electrical properties was observed, indicating their potential as sensors for detecting this analyte. Conclusions: Electrospun PLA/PPy membranes demonstrate high potential for H 2 O 2 detection, owing to their large surface area and high reactivity, thereby enhancing sensor sensitivity. These characteristics make this material a promising option for H 2 O 2 detection applications across various industries.