A portable paper-based aptasensor for simultaneous visual detection of two mycotoxins in corn flour using dual-color upconversion nanoparticles and Cu-TCPP nanosheets

A portable paper-based microfluidic aptasensor is established to simultaneously and visually detect zearalenone (ZEN) and ochratoxin A (OTA). The targets at the sample zone can migrate to two detection zones through dual-channels and result in green and blue fluorescence recovery. This is due to the specific recognition by a respective aptamer that destroys fluorescence resonance energy transfer (FRET) from dual-color upconversion nanoparticles (UCNPs) to Cu-TCPP nanosheets. By capturing fluorescent images and analyzing the corresponding RGB value via a smartphone, ZEN and OTA can be analyzed with limits of detection down to 0.44 ng/mL and 0.098 ng/mL in the linear ranges of 0.5-100 ng/mL and 0.1-50 ng/mL, respectively. Satisfactory recoveries are also obtained for ZEN (94.5-103.7 %) and OTA (92.2-106.8 %) in corn flour. With the advantages of simple operation, low sample consumption, and broad adaptability, this promising platform allows for the on-site detection of multiple hazards in food.