轴突
原位杂交
原位
中期
荧光原位杂交
生物
相间
有丝分裂
分子生物学
DNA
尼克翻译
后期
细胞生物学
细胞周期
细胞
遗传学
染色体
化学
基因表达
基因
再生(生物学)
有机化学
作者
Melissa C. Keinath,Vladimir A. Timoshevskiy
出处
期刊:Methods in molecular biology
日期:2022-10-23
卷期号:: 165-173
标识
DOI:10.1007/978-1-0716-2659-7_10
摘要
Fluorescence in situ hybridization (FISH) is used extensively for visual localization of specific DNA fragments (and RNA fragments) in broad applications on chromosomes or nuclei at any stage of the cell cycle: metaphase, anaphase, or interphase. The cytogenetic slides that serve as a target for the labeled DNA probe might be prepared using any approach suitable for obtaining cells with appropriate morphology for imaging and analysis. In this chapter, we focus on the application of molecular cytogenetic methods such as DNA labeling, slide preparation, and in situ hybridization related to cells from Mexican axolotl.
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