化学
免疫球蛋白E
酶水解
酶
水解
生物化学
抗体
免疫学
生物
作者
Zhiwei Liu,Lin-Lin Zhang,Yingxue Zhou,Panpan Tang,Yen Kheng Tan,Jun‐Hu Cheng,Amin Mousavi Khaneghah,Rana Muhammad Aadil
标识
DOI:10.1016/j.fbio.2022.102161
摘要
Characteristics of cold plasma (CP) treatment and enzymatic (papain and alcalase) hydrolysis on IgG/IgE-binding ability β-lactoglobulin (β-LG) were compared, which is analyzed by immunoblot, WB, ELISA, and LC-MS/MS analysis. Results indicated that CP treatment exhibit similar characteristic to enzymatic hydrolysis to cleave the polypeptides chain of β-LG for reduction of its IgG/IgE binding ability. A similar tendency of decline in IgG/IgE binding capacity of β-LG after CP treatment was achieved in comparison with alcalase hydrolysis, which was reduced by 57.69% (IgG) and 56% (IgE) (for 4 min CP treatment) and 61.89% (IgG) and 27.54% (IgE) for alcalase hydrolysis (75 min), while no significant difference was detected for papain hydrolysis. Immunoblot and LC-MS/MS analysis confirmed that the antigenicity reduction was ascribed to the breakdown of the IgG/IgE linear epitopes of β-LG. Further combined with CP treatment (1 min) with alcalase hydrolysis (75 min), the reduction of antigenicity of β-LG reached 68.46% (IgG) and 59.26% (IgE). This study suggests that CP treatment is a promising alternative to enzymatic hydrolysis for hypoallergenic protein-producing. • CP exhibit similar characteristic to enzyme to cleave β-LG for reducing antigenicity. • CP and Enzyme displayed different selectivity and specificity for cleavage site. • Combined CP and alcalase treatment further reduce the antigenicity of β-LG.
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