脂肪生成
脂肪细胞
脂解
过氧化物酶体增殖物激活受体
调节器
生物
脂肪组织
内科学
罗格列酮
内分泌学
安普克
肌内脂肪
受体
过氧化物酶体
细胞生物学
生物化学
医学
胰岛素
磷酸化
基因
蛋白激酶A
作者
Zhaolu Wang,Youlei Li,Lingling Wu,Yuan Guo,Gongshe Yang,Li Xiao,Xin’e Shi
标识
DOI:10.1080/10495398.2023.2206872
摘要
Intramuscular fat (IMF) positively influences various aspects of meat quality, while the subcutaneous fat (SF) has negative effect on carcass characteristics and fattening efficiency. Peroxisome proliferator-activated receptor gamma (PPARγ) is a key regulator of adipocyte differentiation, herein, through bioinformatic screen for the potential regulators of adipogenesis from two independent microarray datasets, we identified that PPARγ is a potentially regulator between porcine IMF and SF adipogenesis. Then we treated subcutaneous preadipocytes (SA) and intramuscular preadipocytes (IMA) of pig with RSG (1 µmol/L), and we found that RSG treatment promoted the differentiation of IMA via differentially activating PPARγ transcriptional activity. Besides, RSG treatment promoted apoptosis and lipolysis of SA. Meanwhile, by the treatment of conditioned medium, we excluded the possibility of indirect regulation of RSG from myocyte to adipocyte and proposed that AMPK may mediate the RSG-induced differential activation of PPARγ. Collectively, the RSG treatment promotes IMA adipogenesis, and advances SA lipolysis, this effect may be associated with AMPK-mediated PPARγ differential activation. Our data indicates that targeting PPARγ might be an effective strategy to promote intramuscular fat deposition while reduce subcutaneous fat mass of pig.
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