人体皮肤
褪黑素
毛乳头
血清素
色氨酸羟化酶
表皮(动物学)
真皮
角质形成细胞
生物
皮肤癌
黑素细胞
细胞培养
黑色素瘤
细胞
卵泡期
内科学
内分泌学
癌症研究
毛囊
5-羟色胺能
癌症
生物化学
医学
解剖
遗传学
受体
作者
Andrzej Słomiński,Alexander Pisarchik,Igor Semak,Trevor W. Sweatman,Jacobo Wortsman,Andre Szczesniewski,George Slugocki,John A. McNulty,Söbia Kauser,Desmond J. Tobin,Jing Chen,Ӧlle Johansson
标识
DOI:10.1096/fj.01-0952fje
摘要
We investigated the cutaneous expression of genes and enzymes responsible for the multistep conversion of tryptophan to serotonin and further to melatonin. Samples tested were human skin, normal and pathologic (basal cell carcinoma and melanoma), cultured normal epidermal and follicular melanocytes, melanoma cell lines, normal neonatal and adult epidermal and follicular keratinocytes, squamous cell carcinoma cells, and fibroblasts from dermis and follicular papilla. The majority of the samples showed simultaneous expression of the genes for tryptophan hydroxylase, arylalkylamine N-acetyltransferase (AANAT), and hydroxyindole-O-methyltransferase (HIOMT). The products of AANAT activity were identified by RP-HPLC with fluorimetric detection in human skin and in cultured normal and malignant melanocytes and immortalized keratinocytes; HIOMT activity was detected in human skin, keratinocytes, and melanoma cells. N-acetylserotonin (NAS) was detected by RP-HPLC in human skin extracts. NAS identity was confirmed further by LC/MS in keratinocytes. In conclusion, we provide evidence that the human skin expresses intrinsic serotonin and melatonin biosynthetic pathways.
科研通智能强力驱动
Strongly Powered by AbleSci AI