分泌物
微泡
分泌蛋白
高尔基体
细胞生物学
内质网
蛋白质组
生物
分泌途径
细胞外基质
细胞内
蛋白质组学
细胞
信号肽
细胞外
生物信息学
生物化学
肽序列
小RNA
基因
作者
Paul Dowling,Martin Clynes
出处
期刊:Proteomics
[Wiley]
日期:2011-01-13
卷期号:11 (4): 794-804
被引量:129
标识
DOI:10.1002/pmic.201000530
摘要
Abstract In the strictest sense, the cell secretome (conditioned media) refers to the collection of proteins that contain a signal peptide and are processed via the endoplasmic reticulum and Golgi apparatus through the classical secretion pathway. More generally, the secretome also encompasses proteins shed from the cell surface and intracellular proteins released through non‐classical secretion pathway or exosomes. These secreted proteins include numerous enzymes, growth factors, cytokines and hormones or other soluble mediators. They are fundamental in the processes of cell growth, differentiation, invasion and angiogenesis by regulating cell‐to‐cell and cell‐to‐extracellular matrix interactions. The main aim of this review is to provide a synopsis of findings from the analysis of the secretome taking diabetes, cancer and neurodegenerative diseases as examples. We will also discuss the preparation of conditioned media and on the main proteomic‐based methodological approaches that have been developed for the study of secreted/shed proteins.
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