粒体自噬
化学
线粒体
菁
荧光
溶酶体
生物物理学
细胞器
细胞生物学
生物化学
自噬
酶
生物
量子力学
物理
细胞凋亡
作者
Ying Liu,Jin Zhou,Linlin Wang,Xiaoxiao Hu,Xiangjun Liu,Meirong Liu,Zehui Cao,Dihua Shangguan,Weihong Tan
摘要
Mitophagy is a process in which cells remove dysfunctional mitochondria and recycle their constituents in a lysosome-dependent manner. To probe this process, two different fluorescent dyes specific for mitochondria and lysosomes, respectively, are often used in combination. However, current fluorescent dyes for lysosomes cannot distinguish mitochondria-containing autolysosomes from other lysosomes. Therefore, we herein report a cyanine dye, HQO, which can simultaneously probe mitochondria and autolysosomes in live cells by exhibiting different fluorescence properties. HQO selectively accumulates in mitochondria but then transforms to the protonated HQOH(+) form with the decrease of pH when dysfunctional mitochondria evolve into autolysosomes. Since HQO and HQOH(+) exhibit different absorption and emission with Ex/Em at 530/650 and 710/750 nm, respectively, in a low polarity environment, such as that found in micelles, they are uniquely suited to monitor mitophagy with the ability to distinguish autolysosomes from other lysosomes.
科研通智能强力驱动
Strongly Powered by AbleSci AI