病毒学
病毒
免疫分析
核糖核酸
呼吸系统
DNA
生物
基因
抗体
免疫学
遗传学
生物化学
解剖
作者
Huina Wu,Mingyuan Zou,Xuetong Fan,Feiya Su,Xiao Feng,Meiling Zhou,Yuankui Sun,Feng Zhao,Guoqiu Wu
出处
期刊:ACS omega
[American Chemical Society]
日期:2022-04-19
卷期号:7 (17): 15074-15081
被引量:1
标识
DOI:10.1021/acsomega.2c00882
摘要
Influenza viruses and respiratory syncytial virus (RSV) have contributed to severe respiratory infections, causing huge economic and healthcare burdens. To achieve rapid and precise detection of influenza viruses and RSV, we proposed a catalytic hairpin assembly (CHA) combined with the lateral flow immunoassay (CHA-LFIA) detection method. The presence of the target RNA triggers the initiation of CHA circuits. H1/H2 complexes, the amplified signal products, which were labeled with digoxin and biotin, were detected with a highly sensitive lateral flow immunoassay system. The sensitivity of the CHA-LFIA system to influenza A and B viruses and RSV reached up to 1, 1, and 5 pM, respectively. In addition, this method exhibited excellent capability for differentiating between target RNA and base-mismatched RNA. The results demonstrated that an enzyme-free, rapid, highly sensitive, and specific method had been developed to detect influenza A and B viruses and RSV.
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