聚腺苷酸
毛细管电泳
聚合酶
分子生物学
化学
酵母
生物
DNA
信使核糖核酸
生物化学
基因
作者
Deepak P. Patil,Baskar Bakthavachalu,Daniel R. Schoenberg
标识
DOI:10.1007/978-1-62703-971-0_2
摘要
mRNA polyadenylation functions in nuclear export, translation, and stability. We describe an efficient protocol designed to assess poly(A) tail length that is based on 3' tailing by yeast poly(A) polymerase and product analysis to single-nucleotide resolution by capillary electrophoresis.
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