多酚氧化酶
化学
聚丙烯酰胺凝胶电泳
多酚
聚丙烯酰胺
电泳
色谱法
胺气处理
氧化酶试验
染色
凝胶电泳
生物化学
酶
有机化学
高分子化学
生物
抗氧化剂
过氧化物酶
遗传学
作者
Antonio Rescigno,Francesca Sollai,Andrea C. Rinaldi,Giulia Soddu,Enrico Sanjust
出处
期刊:Journal of Biochemical and Biophysical Methods
[Elsevier]
日期:1997-03-01
卷期号:34 (2): 155-159
被引量:23
标识
DOI:10.1016/s0165-022x(96)01201-8
摘要
An analytical method allowing the detection of polyphenol oxidase activity on polyacrylamide gel electrophoresis (PAGE) is described. The method is rapid, sensitive and specific and is based on a coupling reaction between 4-tert-butyl-o-benzoquinone and the aromatic amine, 4-amino-N,N-diethylaniline sulphate. Catecholase activity of polyphenol oxidase appears as blue stained bands on a colourless background.
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