石英晶体微天平
自愈水凝胶
甲基丙烯酸
溶菌酶
生物分子
甲基丙烯酸酯
材料科学
分子质量
高分子化学
蛋白质吸附
丙烯酸
化学工程
生物物理学
化学
吸附
聚合物
纳米技术
生物化学
有机化学
共聚物
复合材料
工程类
生物
酶
作者
Megan S. Lord,Martina H. Stenzel,Anne Simmons,Bruce Milthorpe
出处
期刊:Biomaterials
[Elsevier]
日期:2006-02-01
卷期号:27 (4): 567-575
被引量:126
标识
DOI:10.1016/j.biomaterials.2005.06.010
摘要
Proteins, lipids and other biomolecules interact strongly with the acrylic-based biomaterials used for contact lenses. Although hydrogels are nominally resistant to protein fouling, many studies have reported considerable amounts of protein bound to poly(2-hydroxyethylmethacrylate) (PHEMA) lenses. This study examined the binding of a series of biomolecules (tear protein analogues, mucin and cholesterol) to poly(methylmethacrylate) (PMMA) and three HEMA-based hydrogels (PHEMA, HEMA plus methacrylic acid (P(HEMA-MAA)), HEMA plus methacrylic acid plus N-vinylpyrrolidone (P(HEMA-MAA-NVP))) by use of a quartz crystal microbalance with dissipation (QCM-D) monitoring. The QCM-D estimates changes in the mass and viscous constant for the adsorbed layer through measurements of frequency and dissipation. Protein interaction with each of the test materials caused a net increase in mass of the material indicating protein binding except for lysozyme interacting with P(HEMA-MAA). A net decrease in mass was observed for lysozyme interacting with P(HEMA-MAA) which may be ascribed to lysozyme collapsing the hydrogel by expelling water. A net mass decrease was observed for cholesterol interacting with each of the hydrogel materials, while a mass increase was observed on PMMA.
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