还原胺化
化学
高碘酸盐
琼脂糖
硼烷
胺化
甘氨酸
牛血清白蛋白
琼脂糖
吡啶
色谱法
有机化学
催化作用
生物化学
酶
氨基酸
甘氨酸
作者
Nancy L. Stults,Lisa M. Asta,Y.C. Lee
标识
DOI:10.1016/0003-2697(89)90097-3
摘要
Mild periodate oxidation of certain commercially available crosslinked agarose beads (Sepharose CL-4B and CL-6B) results in the generation of aldehydo groups which were useful for immobilization of amino compounds by reductive amination using pyridine borane. Consumption of periodate ion and production of formaldehyde were only observed with crosslinked Sepharose preparations and were correlated with a binding capacity much greater than that of uncrosslinked gels when subjected to the reductive amination reaction. Up to 50 mg (∼0.73 μmol) of bovine serum albumin and 30 μmol of glycylglycine were coupled per gram of moist oxidized Sepharose CL-6B. The immobilization reaction was shown to proceed at neutral pH requiring about 12 h for completion and to be relatively insensitive to temperature and pyridine borane concentration. The oxidized gel was shown to be stable for at least 2 months upon storage in 0.1 M acetic acid. This method has proven to be useful for the preparation of a variety of affinity matrices and immobilized enzymes.
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