平移(音频)
外周血单个核细胞
单克隆抗体
CD8型
分子生物学
抗体
免疫磁选
化学
生物
细胞毒性T细胞
细胞
免疫学
体外
免疫系统
生物化学
古生物学
缩放
镜头(地质)
作者
Shoshana Morecki,Suzanne L. Topalian,Warren W. Myers,David Okrongly,Thomas B. Okarma,Steven A. Rosenberg
出处
期刊:PubMed
日期:1990-10-01
卷期号:9 (5): 463-74
被引量:14
摘要
A direct positive panning technique was developed in order to achieve highly purified CD4+ and CD8+ cells. Fresh peripheral blood mononuclear cells or tumor-infiltrating lymphocytes derived from bulk cultures were applied to modified polystyrene surfaces to which anti-CD4 or anti-CD8 monoclonal antibodies were covalently attached. Adherent cells were allowed to grow in the original flask and were then harvested and cultured in IL-2-containing medium. This positive immunoselection technique resulted in CD4+ and CD8+ cell subsets with high cell viability and a high degree of purity. In several samples, the isolated cell subsets were subsequently subjected to a negative immunomagnetic bead selection in order to remove reciprocal cell subset contamination or double-positive CD4+/CD8+ cells. The isolated cells maintained their ability to proliferate, kept their phenotypic profiles, and remained functionally intact after long-term growth in culture without the further addition of mitogenic or allogeneic cell stimulation. This approach is a simple, rapid, and reproducible technique that might be useful on a large scale to isolate and to grow T-cell subsets for research and for clinical use.
科研通智能强力驱动
Strongly Powered by AbleSci AI