The preparation of scutellarin from erigeron breviscapus crude extracts was researched.The total flavonoids were got by acid-isolation,from which scutellarin can be well separated by crystallization using methanol as the organic solvent.The optimal conditions were: acid-isolation pH value was 1.5,the yield of total flavonoids can be up to 3.27%,detected by HPLC,the content of scutellarin in the first and second precipitation were 92.0% and 87.9%,respectively,while the content of scutellarin was 94.7% crystalized from methanol.