Isolation and identification of chemical constituents of flavones from the leaves of Acanthopanax senticosus Harms

Objective To investigate the chemical constituents of the extract from the leaves of Acanthopanax senticosus Harms.Methods The chemical constituents were isolated by various isolation methods(silica gel,ODS and HPLC column chromatography)and their structures were elucidated by the analysis of spectral data(NMR and MS).Results Nine flavone compounds were obtained and elucidated as following:isorhamnetin-3-O-robinobioside(1),kaempferol-3-O-robinobioside(2),hyperin(3),isorhamnetin-3-O-β-D-galactopyranoside(4),isorhamnetin-3-O-α-L-rhamnopyranosyl-(1→6)-[α-L-rhamnopyranosyl-(1→2)]-β-D-galactopyranoside(5),kaempferol-3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside(6),quercetin-3-O-α-L-arabinopyaranosyl-(1→2)-β-D-galactopyranoside(7),quercetin(8),kaempferol(9).Conclusions Compounds 1,2,47 are isolated from the Acanthopanax genus for the first time.