Optimization study on water extraction of total flavonoids from alfalfa

To optimize the best water extraction technics of total flavonoids in alfalfa by orthogonal test at different extraction temperature,extraction time and solvent volume conditions. the results showed that the optimal water extraction technics were as follows: extraction temperature was 90 ℃,extraction time was 4 h,and solid to liquid ratio was 1:40. Under this condition the water extraction rate of total flavonoid in alfalfa was 5.960 mg/g.