The strain, UV-63-MW-15 producing less pigment but more pullulan polysaccharide was screened from Aureobasidium pullulans G2m3.44 mutagenized by ultraviolet and microwave. After being immobilized, the strain was inoculated into the starch wasteater added with ground malt root to produce pullulan polysaccharide. The yield of pullulan is higher 26.5% than that of the pullulan polysaccharide produced by the free UV-63-MW-15 strain, and is 2.63 times of that of the pullulan polysaccharide produced by the free original strain.