The Effect of the Wooden Breast Fibrotic Myopathy in Broilers on Fibrillar Collagen Organization and Decorin-Collagen Binding

The wooden breast myopathy is identified by the palpation of a rigid pectoralis major muscle and results in myofiber necrosis and fibrosis in fast-growing, meat-type broilers. The fibrosis in wooden breast-affected muscle is characterized by the replacement of myofibers with extracellular matrix proteins, especially fibril-forming collagens. Studies have shown differences in collagen organization in fast-growing broiler lines, with tightly packed and highly aligned collagen organizations having a higher phenotypic incidence of wooden breast. The objective of the current study was to analyze collagen fibril organization further in two fast-growing broiler lines (Lines A and B) with incidence of wooden breast compared with a slower growing broiler Line C with no phenotypically detectable wooden breast. The small leucine-rich proteoglycan decorin was also studied for its interaction with collagen by immunogold detection. Decorin binds to fibrillar collagens and organizes their alignment and crosslinking, both of which will affect collagen functional properties. Key findings from the study showed that collagen shifts to larger diameter collagen fibril bundles with the wooden breast myopathy. Specifically, broilers affected with wooden breast from Line A had a more dramatic shift toward larger collagen fibril bundles compared with those affected from Line B. Wooden breast-affected Line A had collagen fibril bundles up to 8.4 µm, whereas Line B maximum size was 5.1 µm. Although decorin-collagen binding was not different overall in the wooden breast myopathy or broiler line, for small-diameter collagen fibril bundles, wooden breast-affected Line A had more decorin-collagen binding than wooden breast-affected Line B. Taken together, these data provide further evidence that multiple fibrotic myopathies are likely in fast-growing meat-type broilers.