聚合物
淀粉
超顺磁性
化学工程
生物分子
化学
磁性纳米粒子
结晶
纳米颗粒
粒径
有孔小珠
材料科学
色谱法
纳米技术
有机化学
生物化学
磁化
复合材料
工程类
物理
磁场
量子力学
作者
Ke Luo,Ki-Baek Jeong,Sang-Mook You,Da-Hee Lee,Young-Rok Kim
标识
DOI:10.1021/acs.jafc.8b01590
摘要
Herein, we report a fairly simple and environmentally friendly approach for the fabrication of starch-based magnetic polymer beads (SMPBs) with uniform shape and size through spontaneous rearrangement of short-chain glucan (SCG) produced by enzymatic debranching of waxy maize starch. The paramagnetic materials, dextran-coated iron oxide nanoparticles (Dex@IONPs), were readily incorporated into the starch microstructure and rendered a superparamagnetic property to the SMPBs. The morphology and size of resulting SMPBs turned out to be modulated by Dex@IONPs in a concentration-dependent manner, of which Dex@IONPs was assumed to be acting as a seed inducing the epitaxial crystallization of SCG and further transforming it into homogeneous microparticles. The surface of SMPBs was readily functionalized with an antibody through a one-step reaction using a linker protein. The immuno-SMPBs showed great capture efficiency (>90%) for target bacteria. The colloidal stability and favorable surface environment for biomolecules are believed to be responsible for the high capture efficiency and specificity of the SMPBs. Furthermore, the captured bacteria along with antibody and linker protein were effectively eluted from the surface of SMPBs by adding free maltose, making this new material suitable for various chromatographic applications.
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