The susceptibility of culture cells to severe fever with thrombocytopenia syndrome virus

Objective To learn the susceptibility of culture cells to Severe fever with thrombocytopenia syndrome virus (SFTSV) and strengthen the understanding for transmission and pathogenic mechanism of SFTSV.Method SFTSV infected different cells which derived from 10 cell lines such asVero cells by different multiplicity of infection (MOI).The titration of cell culture supernatant at different time points and the expression of virus antigen were measured by double antibody sandwich ELISA and indirect immunofluorescence assay (IFA).By drawing dynamic growth curve of SFTSV and observing cytopathic effect (CPE) to determine the cell tropism of SFTSV.Result The results suggested that SFTSV can affect Vero,HEK 293,COS-7,CHO,HepG2,Hela,THP-1 and MRC-5,and significant CPE can be observed,but the virus replication levels are significantly different in these cells.Vero cells can relatively susceptible to SFTSV,and the virus titration can reach 1080 TCID50/ml.SFTSV cannot susceptibly affect Raji cells and Jurkat cells.Conclusion SFTSV has broad tissue and cell tropism,and it can infect liver,lung,kidney,uterus,ovaries and other organs as well as the cell lines of immune system,but not T and B lymphocyte-derived cell lines.The study provides important clues to the basic research of SFTSV. Key words: Bunyaviridae ;  Thrombocytopenia;  Physiology ;  Growth