Determination of chlorogenic acid content in beans and leaves of coffea arabica using UV/Vis spectrometer

Chlorogenic acid (CGA) is one of the compounds found in coffee beans and other parts of coffee plant. However, its relative content in different coffee plant parts is least researched. Therefore, this study was designed to determine the contents of CGA in coffee leaves and beans. Green coffee beans and leaves were collected from South part of Ethiopia to study the content of CGA using UV/Vis spectrometer with liquid-liquid extraction method in dichloromethane. Results indicated that mean percentage of CGA in green coffee beans and leaves are in the range of 5.96±0.01 to 6.40±0.02 % and 1.94±0.01 to 2.31±0.01%, respectively. On top of that, the percentage difference of CGA between green coffee beans and leaves was at least about 63%, with beans taking the upper hands. These results showed that there is statistically significant content of CGA in green coffee beans than green coffee leaves. Keywords: CGA, Coffea arabica, coffee leaves, concentration, green coffee beans.