溶剂变色
肉眼
脂滴
荧光
生物安全
化学
香豆素
纳米技术
生物物理学
材料科学
光学
生物化学
生物
有机化学
医学
分子
病理
物理
作者
Jinrong Zheng,Shuheng Qin,Lijuan Gui,Hua Li,Lixue Fan,Yifei Yang,Haiyan Chen,Hui Xu,Zhenwei Yuan
标识
DOI:10.1016/j.cclet.2021.02.059
摘要
Lipid droplets (LDs) are intracellular lipid-metabolism organelles that involved in many physiological processes, metabolic disorders as well as diseases such as atherosclerosis. However, the specific probes that can visually locate abnormal LDs-rich tissues and track LDs-associated behavior to the naked eye with adequate biosafety still are rare. Herein, we develop a new design strategy of LDs-targeted probe based on the solvatochromism of coumarin derivatives. The results revealed that the emission wavelength of coumarin fluorophores gradually red shift in different solvents with increasing polarity, while absorption wavelength almost unchanged. As a result, the enlarged stokes shift of coumarin was emerged from oil to water. Furthermore, properly reducing water solubility and adding electronic donor at the structure of coumarins can enlarge this type of solvatochromism. This discovery was utilized to develop suitable probe for the image of LDs and LDs-rich tissues with high resolution and biosafety. Therefore, LDs-associated behavior was visible to the naked eye during the process of lipophagy and atherosclerosis. We deem that the developed probe here offers a new possibility to accurately diagnosis and analyse LDs-related diseases in clinic and preclinical study.
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