赫拉
转染
细胞内
生物物理学
纳米技术
纳米颗粒
细胞生物学
细胞培养
细胞
化学
材料科学
生物
生物化学
遗传学
作者
Xuan Yang,Xiaowei Wen,Jie Dai,Yanming Chen,Waverly W. Ding,Jun Wang,Xianfeng Gu,Xuejin Zhang,Jin Chen,Roy L. Sutliff,Steven R. Emory,Gang Ruan
出处
期刊:ACS Nano
[American Chemical Society]
日期:2022-05-17
卷期号:16 (6): 8751-8765
被引量:6
标识
DOI:10.1021/acsnano.1c07648
摘要
Hard-to-transfect cells are cells that are known to present special difficulties in intracellular delivery of exogenous entities. However, the special transport behaviors underlying the special delivery problem in these cells have so far not been examined carefully. Here, we combine single-particle motion analysis, cell biology studies, and mathematical modeling to investigate nanoparticle transport in bone marrow-derived mesenchymal stem cells (BMSCs), a technologically important type of hard-to-transfect cells. Tat peptide-conjugated quantum dots (QDs-Tat) were used as the model nanoparticles. Two different yet complementary single-particle methods, namely, pair-correlation function and single-particle tracking, were conducted on the same cell samples and on the same viewing stage of a confocal microscope. Our results reveal significant differences in each individual step of transport of QDs-Tat in BMSCs vs a commonly used model cell line, HeLa cells. Single-particle motion analysis demonstrates that vesicle escape and cytoplasmic diffusion are dramatically more difficult in BMSCs than in HeLa cells. Cell biology studies show that BMSCs use different biological pathways for the cellular uptake, vesicular transport, and exocytosis of QDs-Tat than HeLa cells. A reaction–diffusion–advection model is employed to mathematically integrate the individual steps of cellular transport and can be used to predict and design nanoparticle delivery in BMSCs. This work provides dissective, quantitative, and mechanistic understandings of nanoparticle transport in BMSCs. The investigative methods described in this work can help to guide the tailored design of nanoparticle-based delivery in specific types and subtypes of hard-to-transfect cells.
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