水稻
NADPH氧化酶
生物
平衡
转基因水稻
细胞生物学
盐度
糙米
人口
亚细胞定位
植物
活性氧
生物化学
基因
转基因作物
转基因
食品科学
生态学
人口学
社会学
作者
Qingwen Wang,Lan Ni,Zhenzhen Cui,Jingjing Jiang,Chao Chen,Mingyi Jiang
出处
期刊:Crop Journal
[Elsevier]
日期:2022-12-01
卷期号:10 (6): 1611-1622
被引量:9
标识
DOI:10.1016/j.cj.2022.03.004
摘要
Rice (Oryza sativa L.) is a staple cereal for more than two thirds of the world’s population. Soil salinity severely limits rice growth, development, and grain yield. It is desirable to elucidate the mechanism of rice’s salt-stress response. As the major source of H2O2, NADPH oxidase (Rboh) is believed to be involved in salt-stress tolerance. However, the function and mechanism of rice Rboh in salt stress response remain unclear. In this study, we found that the expression of OsRbohA was up-regulated by NaCl treatment in the shoots and roots of rice seedlings. Knockout of OsRbohA reduced the tolerance of rice to salt stress. Knockout of OsRbohA blocked NaCl-induced increases of NADPH activity and H2O2 content in roots. OsRbohA knockout inhibited root growth and disrupted K+ homeostasis by reducing the expression of K+ transporters and channel-associated genes (OsGORK, OsAKT1, OsHAK1, and OsHAK5) in roots under NaCl treatment. Under NaCl treatment, OsRbohA knockout also reduced subcellular K+ contents of the plasma membrane and soluble fraction. Overexpression of OsRbohA increased the expression of K+ transporters and channel-associated genes and reduced the loss of K+ ions in roots. These results indicate that OsRbohA-mediated H2O2 accumulation modulates K+ homeostasis, thereby increasing salt tolerance in rice.
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