清脆的
细胞外小泡
微泡
基因传递
基因组编辑
纳米技术
计算生物学
输送系统
胞外囊泡
遗传增强
生物信息学
生物
基因
遗传学
细胞生物学
材料科学
药理学
小RNA
作者
Xiaowen Huang,Aifang Li,Ping Xu,Yi‐He Yu,Shuxuan Li,Lina Hu,Shi Feng
标识
DOI:10.1186/s12951-023-01952-w
摘要
Extracellular vesicles (EVs) have emerged as a promising platform for gene delivery owing to their natural properties and phenomenal functions, being able to circumvent the significant challenges associated with toxicity, problematic biocompatibility, and immunogenicity of the standard approaches. These features are of particularly interest for targeted delivery of the emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) systems. However, the current efficiency of EV-meditated transport of CRISPR/Cas components remains insufficient due to numerous exogenous and endogenous barriers. Here, we comprehensively reviewed the current status of EV-based CRISPR/Cas delivery systems. In particular, we explored various strategies and methodologies available to potentially improve the loading capacity, safety, stability, targeting, and tracking for EV-based CRISPR/Cas system delivery. Additionally, we hypothesise the future avenues for the development of EV-based delivery systems that could pave the way for novel clinically valuable gene delivery approaches, and may potentially bridge the gap between gene editing technologies and the laboratory/clinical application of gene therapies.
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