材料科学
分辨率(逻辑)
占空比
荧光
纳米技术
超分辨率
碳纤维
图像分辨率
纳米
光子计数
光子
光学
物理
电压
计算机科学
人工智能
复合数
量子力学
图像(数学)
复合材料
作者
Jayanta Dolai,Prakash Joshi,Partha Pratim Mondal,Anupam Maity,Buddhadev Mukherjee,Nikhil R. Jana
标识
DOI:10.1021/acsami.4c01609
摘要
Single-molecule localization microscopy (SMLM) emerges as a powerful approach for super-resolution imaging that provides unprecedented resolution at the nanometer length scale. However, the development of appropriate probes with specific photophysical traits and characteristics is crucial for this approach. This study demonstrates two different fluorescent carbon dots (CDs) derived from the same molecular precursor─one emitting in red and the other in green─as a SMLM-based super-resolution imaging probe for different applications with an average localization precision of 20 nm and a resolution of 60 nm. Both the CDs exhibit spontaneous blinking with high photon count and low duty cycle but with different blinking cycles. The red emissive CDs with a lower blinking cycle are ideal for quantitative analysis, whereas green emissive CDs with a higher blinking cycle are ideal for high-resolution imaging. We show that the difference in blinking features is linked to their chemical compositions, and the presence of much denser trap states in red emitting CDs is responsible for the reduction of its blinking cycle. This study shows that CDs can be designed as a potential probe for SMLM-based super-resolution imaging for diverse bioimaging applications.
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