Injectable Chitosan–Methoxy Polyethylene Glycol Hybrid Hydrogel Untangling the Wound Healing Behavior: In Vitro and In Vivo Evaluation

Wound healing, particularly for difficult-to-treat wounds, presents a serious threat and may lead to complications. Currently available dressings lack mucoadhesion, safety, efficacy, and, most importantly, patient compliance. Herein, we developed a unique, simple, and inexpensive injectable chitosan–methoxy polyethylene glycol (chitosan–mPEG) hybrid hydrogel with tunable physicochemical and mechanical properties for wound healing. The detailed physicochemical and rheological characterization of the chitosan–mPEG hydrogel has revealed chemical interaction between available −NH2 groups of chitosan and −COOH groups of mPEG acid, which, to our perspective, enhanced the mechanical and wound healing properties of hybrid chitosan and mPEG hydrogel compared to solo chitosan or PEG hydrogel. By introducing mPEG, the wound healing ability of hydrogel is synergistically improved due to its antibacterial feature, together with chitosan’s innate role in hemostasis and wound closure. The detailed hemostasis and wound closure potential of the chitosan–mPEG hydrogel were investigated in a rat model, which confirmed a significant acceleration in wound healing and ultimately wound closure. In conclusion, the developed chitosan–mPEG hydrogel met all the required specifications and could be developed as a promising material for hemostasis, especially wound management, and as an excellent candidate for wound healing application.