DNA甲基化
细胞生物学
拟南芥
生物
转录因子
分生组织
甲基化
表观遗传学
干细胞
遗传学
DNA
基因表达
基因
突变体
作者
Jian Zeng,Xiaoying Zhao,Zhe Liang,Inés Hidalgo,Michael Gebert,Pengfei Fan,Christian Wenzl,Sebastian G. Gornik,Jan U. Lohmann
标识
DOI:10.1038/s41467-023-43705-1
摘要
Abstract Despite the importance of Nitric Oxide (NO) as signaling molecule in both plant and animal development, the regulatory mechanisms downstream of NO remain largely unclear. Here, we show that NO is involved in Arabidopsis shoot stem cell control via modifying expression and activity of ARGONAUTE 4 ( AGO4 ), a core component of the RNA-directed DNA Methylation (RdDM) pathway. Mutations in components of the RdDM pathway cause meristematic defects, and reduce responses of the stem cell system to NO signaling. Importantly, we find that the stem cell inducing WUSCHEL transcription factor directly interacts with AGO4 in a NO dependent manner, explaining how these two signaling systems may converge to modify DNA methylation patterns. Taken together, our results reveal that NO signaling plays an important role in controlling plant stem cell homeostasis via the regulation of de novo DNA methylation.
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