银染
染色
聚丙烯酰胺
聚丙烯酰胺凝胶电泳
蛋白质检测
化学
色谱法
材料科学
纳米技术
生物化学
分子生物学
生物
高分子化学
遗传学
酶
作者
Lijing Zhao,Cong Li,Yinpeng Sun,Liping Ban
标识
DOI:10.1002/elps.201200107
摘要
Silver staining is widely used to detect protein in polyacrylamide gels when high sensitivity is required. A simple and rapid protocol for silver staining of proteins following PAGE was developed in the present study. The number of steps was reduced compared to conventional protocol by combining fixing, rinsing, and soaking into a single impregnating step, thus achieving detection of proteins in 20 min. The present method is as sensitive as current protocols with the advantage of saving time and costs.
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