化学
电喷雾电离
串联质谱法
色谱法
试剂
离子
离子色谱法
串联
质谱法
光化学
有机化学
材料科学
复合材料
作者
Lin Zhang,Gargey Yagnik,Dianlu Jiang,Shuyun Shi,Peter R. Chang,Feimeng Zhou
标识
DOI:10.1016/j.jchromb.2012.10.026
摘要
Reversed-phase ion-pairing chromatography (RP-IPC) is coupled on-line with electrospray ionization-mass spectrometry (ESI-MS) through an interface comprising a four-way switch valve and an anion exchange column. Regeneration of the anion exchange column can be accomplished on-line by switching the four-way switch valve to interconnect the column to a regeneration solution. Positioning the anion exchange column between the RP-IPC and ESI-MS instruments allows the ion-pairing reagent (IPR) sodium octane sulfonate to be removed. The IPC-ESI-MS method enabled us to separate and detect four intermediates of the Fe(III)-catalyzed dopamine oxidation. In particular, 6-hydroxydopamine, which is short-lived and highly neurotoxic, was detected and quantified. Together with the separation of other intermediates, gaining insight into the mechanism and kinetics of the Fe(III)-catalyzed dopamine oxidation becomes possible.
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