Construction and preliminary selection and identification of phage display library of anti-human ovarian cancer single chain Fv antibody

AIM: To construct the phage display library of anti human ovarian cancer single chain Fv antibody. METHODS: The ScFv gene library was constructed by phage display technology. The affinity to antigen was detected by ELISA after panning. RESULTS: Twenty six antigen binding clones were identified from 94 individual phagemid clones. CONCLUSION: In the production of engineering antibodies against human cancer, this strategy may provide an alternative approach.